Anti-BRG1/SMARCA4 (IHC)

Anti-BRG1/SMARCA4 (IHC)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
IHC-00466-T 10 µl (5+ slides) -

3 - 9 business days*

131.00€
IHC-00466 100 µl (50+ slides) -

3 - 9 business days*

493.00€
 
Protein function: Transcriptional coactivator cooperating with nuclear hormone receptors to... more
Product information "Anti-BRG1/SMARCA4 (IHC)"
Protein function: Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self- renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. [The UniProt Consortium]
Keywords: Anti-SMARCA4, Anti-BAF190A, Anti-SNF2-beta, EC=3.6.4.-, Anti-Protein BRG-1, Anti-Protein brahma homolog 1, Anti-BRG1-associated factor 190A, Anti-Transcription activator BRG1, Anti-ATP-dependent helicase SMARCA4
Supplier: Bethyl Laboratories
Supplier-Nr: IHC-00466

Properties

Application: IHC
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human, Mouse (Expected: Dog, Horse, Rabbit, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Duckbill Platypus, White-tufted-ear Marmoset, Crab-eating Macaque, European Domestic Ferret, African Elephant, Northern White-cheeked Gibbon)
Immunogen: synthetic peptide. The epitope recognized by IHC-00466 maps to a region between residue 75 and 125 of human BRG1/SMARCA4 using the numbering given in entry NP_003063.2 (GeneID 6597).
Format: Antigen Affinity Purified

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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