Product information "Anti-beta-Lactamase, clone 1.BB.875"
The beta-lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta-lactams are structurally related through the presence of a core beta-lactam ring. Bacterial resistance to beta-lactams continues to increase, primarily due to the production of beta-lactamases. Beta-lactamases catalyze the hydrolysis of the beta-lactam bond which destroys antibacterial activity. Bacteria that produce TEM-or SHV-type beta-lactamases have point mutations in structural genes that have extended the substrate speci?city of these beta-lactamases. As a result, many of the beta-lactamase-producing Gram-negative pathogens have become multi-drug resistant. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 10-20ug/ml, Western Blot: 10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.