Anti-BACE1 (26-45) (Beta-site APP Cleaving Enzyme)

b-secretase, also called BACE (b-site of APP cleaving enzyme), is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease and in the formation of myelin sheaths in peripheral nerve cells. BACE1, also known as memapsin-2, is the major b-secretase for generation of the 40 or 42 amino-acid-long b-amyloid peptides that aggregate in the brain of Alzheimer's patients. Extracellular cleavage of APP by BACE1 releases a soluble extracellular fragment and is followed by APP cleavage within its transmembrane domain by g-secretase. The second cleavage releases the intracellular domain of APP and b-amyloid., The immature form of BACE1 contains an N-terminal signal sequence (residues 1-21) followed by a large catalytic domain, a single transmembrane domain (residues 461-477), and a short cytoplasmic domain (residues 478-501). The signal sequence (1-21) is cleaved from the immature form, yielding the proBACE1 protein, which starts at residue 22. The proBACE1 protein is modified by cleavage of 12 N-terminal residues (aa 22-45), producing the mature BACE1 protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.1-1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.