Anti-ATP12A, ID (Potassium-transporting ATPase alpha Chain 2, Proton Pump, Non-gastric H(+)/K(+) ATP

ATP12A belongs to the family of P-type cation transport ATPases. This protein is a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Applications: Suitable for use in FLISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: FLISA: 1:1000, Flow Cytometry: 1:10:1:50, Immunohistochemistry (paraffin): 1:50-1:100, Western Blot: 1:100-1:500, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap., , Note: Applications are based on unconjugated antibody.