Anti-ASIALOGLYCOPROTEIN RECEPTOR (ASGPR)

The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42kD) which mediates binding, internalisation and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i.e. it is present on the sinusoidal and lateral plasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediates the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, Western Blot: 1:50 Detects a band at ~42kD. A non-reduced sample treatment and SDS-PAGE followed by blotting on nitrocellulose or PDFM is recommended. Block with 10% non-fat dry milk., Immunohistochemistry (frozen): 1:50 Acetone-fixed, ELISA: 20ug/ml using 100ul/well, Optimal dilutions to be determined by the researcher. Recommended Positive Control: Rat hepatocytes, Recommended Negative Control: Rat Kidney, pancreas, small intestine, colon, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.