Anti-AMP Activated Protein Kinase alpha1/2 (AMPK a1/2)

AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilized by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels., AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:1000 Detects a band at ~63kD in rat brain cell lysates., Immunohistochemistry: Frozen, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.