Anti-ALPP / Alkaline phosphatase placental, clone MSVA-350R

Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide. Alkaline phosphatase placental is a glycosylphosphatidylinositol-anchored membrane enzyme encoded by the ALPP gene and commonly referred to as placental alkaline phosphatase or PLAP. The ALPP Antibody for IHC is developed to evaluate tissue distribution and membranous expression of this trophoblast-associated isoenzyme in formalin-fixed, paraffin-embedded specimens. ALPP is located on chromosome 2q37 and belongs to the alkaline phosphatase family of homodimeric metalloenzymes that hydrolyze phosphate monoesters under alkaline conditions.In normal tissue, placental alkaline phosphatase is strongly expressed on the apical membrane of syncytiotrophoblasts, producing a distinct membranous staining pattern in placental tissue. Outside of pregnancy-associated tissues, ALPP expression is limited, which supports its utility in immunohistochemical panels assessing trophoblastic differentiation. The glycosylphosphatidylinositol anchor localizes the enzyme to the external surface of the plasma membrane, resulting in crisp membranous staining in positive epithelial or trophoblastic cells.In tumor pathology research, ALPP is widely recognized as a marker of germ cell tumors, particularly seminoma and embryonal carcinoma, as well as certain trophoblastic neoplasms. In these contexts, strong membranous and occasionally cytoplasmic staining is observed in tumor epithelial cells, while most non-germ cell malignancies show minimal or absent staining. This restricted distribution pattern enhances its value in studies examining lineage differentiation and tumor classification within tissue sections.Although ALPP shares structural similarity with other alkaline phosphatase isoenzymes, including tissue-nonspecific and intestinal alkaline phosphatases, its expression profile is more restricted and lineage-associated. Evaluation of ALPP expression by immunohistochemistry supports research focused on trophoblastic biology, germ cell tumor characterization, and membrane-associated enzyme localization within histologic architecture. Clone MSVA-350R is a recombinant rabbit monoclonal antibody developed for detection of ALPP in tissue-based research applications.This antibody is also part of a broader collection of IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.