Anti-ALOX15 (Arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2)

Anti-ALOX15 (Arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
A1359-80.100 100 µg - -

3 - 19 business days*

Applications:|Suitable for use in ELISA and Western Blot. Other applications not... more
Product information "Anti-ALOX15 (Arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2)"
Applications:, Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:32,000. , Western Blot: 0.2-0.5µg/ml. Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Optimal dilutions to be determined by the researcher. Storage and Stability: , May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer., , Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See A1359-80-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Supplier: United States Biological
Supplier-Nr: A1359-80


Application: ELISA, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Goat
Reactivity: Human
Immunogen: A synthetic peptide from the C-terminus of aa-HYKTDVAVKDDPE.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Our products are for laboratory research use only: Not for administration to humans!
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