Anti-15-hydroxy Prostaglandin Dehydrogenase

NAD+-dependent 15-PGDH catalyzes the oxidation of prostaglandins to 15-keto metabolites, which have greatly reduced biological activity. Human NAD+-dependent 15-PGDH is a 266 amino acid protein with a molecular weight of 29 kDa and is expressed in multiple tissues including lung, placenta, and kidney. The enzyme belongs to a large family of short-chain alcohol dehydrogenases, which exhibit amino acid identity ranging from 15-30%. An NADP+-dependent 15-PGDH, which is also part of this enzyme family, exhibits only 20% amino acid identity to the NAD+-dependent enzyme. The peptide used as the antigen for preparation of the 15-PGDH antibody is highly conserved across several species (see above), but has only minor homology to other proteins within the short-chain alcohol dehydrogenase family of enzymes (only the alanine and glycine at the N-terminus of the sequence appear to be conserved). Cayman's 15-PGDH Polyclonal Antibody can be used for Western blot applications. The antibody recognizes 15-PGDH at 29 kDa from human, baboon, and mouse samples.Synonyms: 15-PGDH. Immunogen: Synthetic peptide from an internal region of human 15-PGDH. Formulation: (Request formulation change), Peptide affinity-purified polyclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Rabbit. Isotype: IgG. Applications: WB. UniProt Accession #: P15428. Origin: Animal/Rabbit.