Anti-GFP (Green Fluorescent Protein) (APC)

AB The Green Fluorescent Protein (GFP), originally identified and isolated from the jellyfish Aequorea victoria, is a universally used fluorescent indicator, utilized as a reporter for gene expression, a cell lineage tracer, and for measurements of protein-protein interactions. GFP and chimeric proteins fused to GFP have been expressed in virtually all types of cell and are used as non-invasive fluorescent markers in living cells and organisms. GFP is ~27kD and 238aa and emits green light when excited with blue or UV light (emission peak at 509nm and excitation peak at 395nm). Given its widespread use and potential applications, several GFP mutants have been developed, including enhanced GFP (EGFP), commonly used in mammalian cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul Labels 1x10e6 cells, Optimal dilutions to be determined by the researcher. Absorption: 620-650nm , Emission: 660-670nm, Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Freezing Labeled with Allophycocyanin (APC) conjugates will result in a substantial loss of activity. Further dilutions can be made in assay buffer. APC is light sensitive.