Purification Media, Affinity, Protein A

Purification Media, Affinity, Protein A
Item number Size Datasheet Manual SDS Delivery time Quantity Price
P9100-70.10 10 mg - -

3 - 19 business days*

335.00€
P9100-70.50 50 mg - -

3 - 19 business days*

771.00€
 
Recombinant Protein-A used also in Protein A beads (P9100-75) is a genetically engineered form of... more
Product information "Purification Media, Affinity, Protein A"
Recombinant Protein-A used also in Protein A beads (P9100-75) is a genetically engineered form of Protein-A derived from Staphylococcus aureus. Non-essential regions have been modified while leaving the IgG binding sites intact. Protein A is been used extensively used for the isolation of IgG from several species of mammals. The affinity of Protein A to IgG's is different between species and IgG subclasses within a given species. A major known limitation of protein A is its weak binding to Mouse IgG1- a major immunoglobulin subclass in Mice. Despite its variable binding characteristics, Protein A is a popular choice for both antibodies binding in various assays and when coupled to beads, for Immunoprecipitation of -antigen complexes. Protein A binds specifically the Fc region of immunoglobulins leaving the Fab region free for binding the antigen. Hence, Protein A is extremely useful for isolating of immune complexes. Protein A Beads characteristics: , Source:, Recombinant protein, Molecular Weight: 23.3kD expressed in E. coli, Purity: > 90% by SDS-PAGE, Spectroscopic analysis: OD280nm/250nm=2.51, Endotoxin: < 0.5EU/mg Recombinant Protein A (LAL), Composition: Lyophilized, white powder. 10.9mg Recombinant Protein A, 0.82 mg, phosphate buffer salts. Storage before Reconstitution: Store at 4°C. Product is stable under these conditions for one year. Storage after Reconstitution: Store at 4°C in PBS pH 7.4 added with sodium azide 0.1% (w/v) as a preservative. Product is stable under these conditions for 1 month. Alternatively, prepare small aliquots and store at -20°C for up to two years. Avoid repeated freezing and thawing cycles. , Storage and Stability:, Stable at RT for 1 week
Supplier: United States Biological
Supplier-Nr: P9100-70

Properties

Application: Antibody isolation

Database Information

Handling & Safety

Storage: +4°C
Shipping: +20°C (International: +20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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