Purification Media, Affinity, Epoxy

Epoxy-Activated Beads is a preactivated support that contains high density of epoxy groups used for immobilization of various ligands. The preactivated support can be used to immobilize proteins, carbohydrates and various ligands via stable linkage to amino, thiol and hydroxyl groups. Coupling to hydroxyls is favored at a higher pH of 11-13, while Thiol-containing ligands are best coupled at pH 7.5-9.0., , Epoxy-Activated Beads characteristics:, Matrix: Sepharose CL-4B, Active group: Oxiran, Active group density: 10-25umole/ml, Bead size: 45-165um, Bead structure: Highly cross-linked spherical agarose, 6%, Max back pressure: 0.3MPa, 3 bar, Max. flow rates: 4ml/min/cm2, Recommended flow rate: 1-2 ml/min/cm2, Stability of the matrix: pH 2-11, Storage: Store gel refrigerated in phosphate buffered saline (PBS) or other ligand-compatible, buffer added with 0.1% azide (w/v) as a preservative until use.