Actin Polymer Buffer (10X)

Actin Polymer Buffer (10X)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
PUR-000103-5 5 ml -

4 business days*

136.00€
 
Actin Polymer Buffer (10X) is comprised of 500 mM KCl and 20 mM MgCl2. Actin Polymer Buffer (10X)... more
Product information "Actin Polymer Buffer (10X)"
Actin Polymer Buffer (10X) is comprised of 500 mM KCl and 20 mM MgCl2. Actin Polymer Buffer (10X) supports the polymerization of G-actin into F-actin in vitro. Polymerization Protocol: Dilute Ultra-Pure Actin (PUR-160101) to 0.4 mg/ml with Actin Working Buffer (PUR-000102, 5 mM Tris-HCl and 0.2 mM CaCl2, pH 8.0) supplemented with 0.2 mM ATP and 1 mM DTT. Incubate on ice for 1 hour followed by centrifugation at 14k rpm for 15 minutes at 4°C. Collect the supernatant and add 1/10 volume of Actin Polymer Buffer (10X). Incubate at room temperature for 1 hour to polymerize. The resulting actin filaments can be stored at 4°C for 2 weeks with the addition of 0.05% sodium azide.
Supplier: PurSolutions
Supplier-Nr: 000103

Properties

Format: Solution

Database Information

Handling & Safety

Storage: +4°C
Shipping: +20°C (International: +20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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