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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
PUR-000103-5 | 5 ml | - | 4 business days* | 136.00€ |
You can also order using info@biomol.com or FAX at 0049-40-85326022
Bigger quantity required? Request Bulk
Bigger quantity required? Request Bulk
Actin Polymer Buffer (10X) is comprised of 500 mM KCl and 20 mM MgCl2. Actin Polymer Buffer (10X)... more
Product information "Actin Polymer Buffer (10X)"
Actin Polymer Buffer (10X) is comprised of 500 mM KCl and 20 mM MgCl2. Actin Polymer Buffer (10X) supports the polymerization of G-actin into F-actin in vitro. Polymerization Protocol: Dilute Ultra-Pure Actin (PUR-160101) to 0.4 mg/ml with Actin Working Buffer (PUR-000102, 5 mM Tris-HCl and 0.2 mM CaCl2, pH 8.0) supplemented with 0.2 mM ATP and 1 mM DTT. Incubate on ice for 1 hour followed by centrifugation at 14k rpm for 15 minutes at 4°C. Collect the supernatant and add 1/10 volume of Actin Polymer Buffer (10X). Incubate at room temperature for 1 hour to polymerize. The resulting actin filaments can be stored at 4°C for 2 weeks with the addition of 0.05% sodium azide.
Supplier: | PurSolutions |
Supplier-Nr: | 000103 |
Properties
Format: | Solution |
Database Information
Handling & Safety
Storage: | +4°C |
Shipping: | +20°C (International: +20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
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