DNA Marker 1kb Ladder, 14 Fragments (250-10,000bp), Ready to Use, BioAssay

DNA Marker 1kb Ladder is designed for sizing and approximate quantification of wide range double-stranded DNA fragments on agarose gel. The ladder is composed of fourteen chromatography-purified individual DNA (in base pairs): 10000, 8000, 6000, 5000, 4000, 3500, 3000, 2500, 2000, 1500, 1000, 750, 500, 250. It contains three reference bands . It contains three reference bands of (6000, 3000 and 1000bp) for easy orientation. 6X Loading Dye Solution L3350-01: (Included) Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 0.15% orange G, 60mM EDTA, 60% glycerol. Band Concentration: 0.5ug of DNA Ladder Mix contains:, a) 30ng (6.0%) for each of the 3500, 4000, 5000, 8000 and 10,000bp reference fragments., b) 70ng (14.0%) for 3000, 6000 bp bands , c) 60ng (12.0%) for 1000 bp band, d) 25ng (5.0%) for 250-750 and 1500-2500 bp bands, Protocol: 1. Vortex gently prior to use, 2. Load 1ul/1mm gel lane. Recommendations for Use: 1. Vortex gently just prior to use. 2. Do not heat before loading. , 3. Dilute DNA sample with L3350-01, 6X DNA Loading Dye, mix 1 volume of dye with 5 volumes of DNA sample. 4. Load same sample volumes of the DNA sample and the ladder. , 5. For quantitation, adjust the concentration of the sample to match the amount of DNA in the nearest band of the ladder. 6. Visualize DNA bands by staining with intercalating dyes. Do not add the dyes into the sample. Use gel staining after electrophoresis or include dyes into agarose gel to avoid aberrant DNA migration. , Storage and Stability:, May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.