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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
N1000.500 | 500 g | - | - |
3 - 19 business days* |
142.00€
|
||
N1000.1 | 1 kg | - | - |
3 - 19 business days* |
274.00€
|
A standard preparation for use in Nematode growth studies.||Appearance: |Light tan to pale... more
Product information "Nematode Growth Medium (NGM) (Powder)"
A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder, Solubility without autoclaving: Light tan, incomplete, Solubility with autoclaving: Light tan, clear, complete, pH with autoclaving: As Reported, Protocol: Cooling the N1000 solution after autoclaving: , Place the sterile N1000 solution into a 58°C water bath. N1000 solution needs to completely cool to the desired temperature (58°C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58°C, place flask(s) onto stir plate(s). Maintain temperature at 58°C. Add the following:, a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution, b)Sterile 1M CaCl2: 1ml/liter N1000 solution, c) Sterile 1M MgSO4: 1ml/liter N1000 solution, Allow the solution to mix for five minutes before starting to pour plates to ensure that a homogeneous mixture has been achieved., *1M Phosphate buffer is prepared by dissolving 108.3g KH2PO4 and 35.6g K2HPO4 in ddH2O. QS to 1L).
Supplier: | United States Biological |
Supplier-Nr: | N1000 |
Properties
Format: | Molecular Biology Grade |
Database Information
Handling & Safety
Storage: | vT |
Shipping: | +20°C (International: +20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
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