HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. It is one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by...

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. (www.uniprot.org)...

Pefabloc® SC is a water soluble, irreversible serine protease inhibitor. It inhibits proteases like chymotrypsin, kallikrein, plasmin, thrombin, and trypsin. The specificity is similar to the inhibitor PMSF, nevertheless Pefabloc® SC is more stable at low pH values. Store stock solutions (100 mM in water) for up to...

Induces cartilage and bone formation. (www.uniprot.org) Produced in E.coli. Homodimeric, non-glycosylated polypeptide chain containing 115 amino acids. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less then 50 ng/ml.

DMSO is a polar aprotic solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water. DMSO is less toxic than other members of this class, such as dimethylformamide, dimethylacetamide, N-methyl-2-pyrrolidone, and HMPA. DMSO is also used in PCR to...

Specially designed for western blotting using IRDye conjugated antibodies. Composed of ultra pure reagents and a proprietary protein formulation in Tris pH 7.6.

Bovine Serum Albumin (BSA), Fraction V is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screenining assays, western blotting, and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as...

Chromomycin A3 is an anthraquinone antibiotic and antitumor agent isolated from S. griseus that is used as a fluorescent probe for DNA with excitation/emission spectra of 445/575 nm. Its DNA binding is specific to two or more contiguous GC base pairs, which makes it suitable for characterizing heterochromatin in...

Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality...

For 100 applications. Contains a unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs....

Bicine is an organic buffering agent. It is one of Good's buffers and has a pKa of 8.35 at 20°C. It is prepared by the reaction of glycine with ethylene oxide, followed by hydrolysis of the resultant lactone. Useful pH range: 7.8-8.8

Leupeptin, also known as N-acetyl-L-leucyl-L-leucyl-L-argininal, is a naturally occurring protease inhibitor that can inhibit cysteine, serine and threonine peptidases. It is often used during in vitro experiments when a specific enzymatic reaction is being studied. When cells are lysed for these studies, proteases,...