DNA Marker BsuRI (HaeIII), 22 Fragments (8-587bp), BioAssay (Supplied with 6x Loading Dye Solution)

pBR322 DNA was completely digested by BsuRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 22 discrete fragments (in base pairs): 587, 540, 502, 458, 434, 267, 234, 213, 192, 184, 124, 123, 104, 89, 80, 64, 57, 51, 21, 18, 11, 8. Concentration: 0.5mg DNA/ml , Storage Buffer: 10mM Tris-HCl (pH 7.6), 1mM EDTA, 6X Loading Dye Solution: 0.09% bromophenol blue, 0.09% xylene cyanol, 60% glycerol, 60mM EDTA. Quantity of DNA in band:, Fragment (bp) % ng DNA 587 13.4 134, 540 12.4 124, 504 11.6 116, 458 10.5 105, 434 10.0 100, 267 6.1 61, 234 5.3 53, 213 4.9 49, 192 4.4 44, 184 4.2 42, 124 2.8 28, 123 2.8 28, 104 2.4 24, 89 2.0 20, 80 1.8 18, 64 1.5 15, 57 1.3 13, 51 1.2 12, 21 0.5 5, 18 0.4 4, 11 0.3 3, 8 0.2 2