GenieClone 5 Minute Ligation Mix

GenieClone 5 Minute Ligation mix is a ready-to-use 2 x premix solution suitable for sticky-end ligation, blunt-end DNA ligation and TA ligation. The kit contains an optimised T4 DNA ligase that catalyzes the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini in double-strand DNA or RNA. The unique buffer system comes with optimized ligation enhancer making the reaction much more efficient and convenient. Ligation can be completed in 5 min at 25?. The GenieClone 5 Minute Rapid Ligation mix can be used directly to transform many types of chemically competent cells. Applications: * Sticky end ligation * Blunt end ligation * TA ligation * Linker or Adapter ligation