Human B Cell Isolation Kit

The Human B Cell Isolation Kit delivers a high purity population of human B cells that is ready for downstream use. The BACSTM Microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of immune cells during enrichment., , Mechanism of Action: The Human B Cell Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich B cells. Non-B cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD2, CD3, CD14, CD15, CD16, CD36, CD43, CD56, CD57, CD123, CD161, CD235ab, and CD314., , How it works: The biotinylated antibody cocktail is added to the sample to label unwanted cells. BACSTM Microbubbles are added to the sample and bind to the antibody-labeled cells. Microbubbles bound to the undesired cells, quickly and gently float the undesired cells to the surface. B cells, the cells of interest, are pelleted through centrifugation. Microbubble-bound cells and supernatant are easily aspirated away, leaving untouched and enriched B cells healthy and ready for downstream use., , . Cell Type: Human B Cells, , . Sample Species and Source: Human MNCs, , . Cell Separation Method: Negative selection, , . Capacity: 1 billion MNCs, , Kit Includes:, , . BACSTM Streptavidin Microbubbles, , . Human B Cell Biotin Antibody Cocktail, , . Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA), , . 5 ml Tubes