Transmembrane Buffer (10×)

Tris-Glycine transmembrane buffer is used in the wet and semi-dry test of Western blotting experiments. The protein can be transferred from the gel to the PVDF membrane or the NC membrane. This product is supplied with 10 × concentrate for easy transportation (without methanol). Dilute with pure water and methanol to 1 × working solution before use. For example: take 100 mL Transmembrane Buffer (10 ×), 200 mL methanol,dilute with pure water to 1 L. 1 × Transmembrane Buffer Components: 19.6 mM Tris-base, 150 mM Glycine, 20% (v/v) Methanol, pH8.3.