5 × SDS Loading Buffer

This product is a loading buffer for protein samples with SDS-PAGE electrophoresis. The SDS contained in the product can be combined with the protein to form a SDS-protein complex, which bring a large amount of negative charge to the protein. SDS can break intramolecular and intermolecular hydrogen bonds, and destroy the secondary and the tertiary structure of protein. The DTT contained in the preparation can break the disufide bond between the cysteine residues, destroy the structure between the proteins, and eliminate the difference between the protein structures. Ultimately, the rate of protein migration in the SDS-PAGE is only related to its molecular weight. Bromophenol blue is used as an indicator for electrophoresis to determine the progress of electrophoresis.