Insulin Protein (recombinant human)

Activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells.The ED50 for this effect is typically 0.2-1 µg/mL. Protein Construction: A DNA sequence encoding human insulin B chain (2LGB_B)(Phe1-Lys29) and human insulin A chain (2KXK_A)(Gly1-Asn21) was expressed with a linker AAK. Sequence: Phe1-Lys29. Endotoxin: Please contact us for more information. Apparent Molecular Mass: 6 kDa. Protein function: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy Protein function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [The UniProt Consortium]