Insulin-Like Growth Factor Binding Protein, active 3, active (IGFBP3), Recombinant, Human

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. {ECO:0000269, PubMed:20353938}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200ng/ml, corresponding to a specific activity of ?5.0x10e3 IU/mg in the presence of 15ng/ml of rHuIGF-II. Sequence: GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK, Molecular Weight: 28.8kD, PubMed ID: 2464130, 1695633, 7508771, 12853948, 12690205, 15489334, 1697583, 1726837, 15340161, 14561895, 14760718, 16335952, 19159218, 19139490, 20353938, 260910e39, 16959974, Gene Name: IGFBP3, IBP3, Swiss Prot: P17936, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.