CD40L (human) (multimeric)

(3 x 10 µg). Human CD40L (amino acids 116-261) is fused at the N-terminus to mouse ACRP30headless (amino acids 18-111) and a FLAG(R)-tag. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml). Lyophilized. Contains PBS MultimericCD40L is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.