Fetuin alpha-2 Heremans-Schmid Glycoprotein (FHSG, Fetuin A)

Fetuin is a liver-produced negative acute phase protein composed of two subunits, the A and B chains Fetuin homologs have been identified in several species including rat, sheep, pig, rabbit, guinea pig, cattle, mouse and human. Multiple physiological roles for these homologs have been suggested, including ability to bind to hydroxyapatite crystals and to specifically inhibit the tyrosine kinase (TK activity of the insulin receptor (IR). Fetuin-A (alpha2-Heremans-Schmid glycoprotein, AHSG) is an important circulating inhibitor of calcification in vivo, and is downregulated during the acute-phase response. Sera from patients on longterm dialysis with low AHSG concentrations showed impaired ex-vivo capacity to inhibit CaxPO4 precipitation. Fetuin may influence the resolution of inflammation by modulating the phagocytosis of apoptotic cells by macrophages. ASHG blocks TGF-beta-dependent signaling in osteoblastic cells, and, mice lacking ASHG display growth plate defects, increased bone formation with age, and enhanced cytokine-dependent osteogenesis. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Reconstitution: Reconstitute in 0.2ml sterile ddH2O. Allow the lyophilized pellet to dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the protein. Clarify by centrifugation. , Storage and Stability:, Lyophilized powder may be stored at 4°C for up 2 weeks only. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store reconstituted protein at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.