T4 DNA ligase

T4 DNA Ligase is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 4x Reaction Buffer, which includes ATP. T4 DNA Ligase catalyses the formation of a phosphodiester bond between juxtaposed 5'-phosphoryl and 3'-hydroxyl termini in duplex DNA. It repairs single-strand nicks in duplex DNA and will join both blunt and cohesive-end restriction fragments of duplex DNA or RNA. The enzyme requires ATP as cofactor. Applications: - Cloning of both blunt- and sticky (cohesive)-end restriction fragments - Cloning of PCR products - Joining linkers and adapters to DNA - Nick repair - Self-circularization of linear DNA. Components: * T4 DNA Ligase (5 U/µL) * Reaction buffer (4x). Shipping Conditions: Dry Ice. Storage Conditions: -85 °C to -15 °C. Activity: Blunt-end ligation, Sticky-end ligation. Product Category: Ligases. Reaction Temperature: 16 - 20 0C. Heat Inactivation: 65 °C. Concentration: 5 U/µL