ST2 Protein (isoform a, His Tag) (recombinant human)

Activity: Measured by its binding ability in a functional ELISA.2. Immobilized recombinant human IL33 at 10 µg/mL (100 µl/well) can bind biotinylated human IL1RL1 (isoform A) with a linear range of 0.032-8 µg/ml. Protein Construction: A DNA sequence encoding the human IL1RL1 isoform A (NP_057316.3) (Met 1-Ser 328) was expressed, fused with a polyhistidine tag at the C-terminus. Sequence: Met 1-Ser 328. Fusion tag: C-His Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method. Protein function: IL-1 receptor-like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interlukin-1 like 1 (IL1RL1) protein can be used to diagnose cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described, and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage pro-inflammatory function. IL33 is a specific ligand of ST2L and induces production of Th2 cytokines. Protein function: Receptor for interleukin-33 (IL-33), signaling requires association of the coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. [The UniProt Consortium]