PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)

PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
P4278-01.25 25 µg - -

3 - 19 Werktage*

886,00 €
 
Mouse PLTP Phospholipid transfer protein (PLTP, also lipid transfer protein II) is an 80kD,... mehr
Produktinformationen "PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)"
Mouse PLTP Phospholipid transfer protein (PLTP, also lipid transfer protein II) is an 80kD, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily, BPI/LBP family of molecules (1). It is synthesized as a precursor with a 17aa signal sequence and a 476aa mature chain (SwissProt # P55065). The mature chain contains two BPI/LBP/CETP domains (aa21-239 and 263-463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4-6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha-tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7-11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6). Source: A DNA sequence encoding the mature mouse PLTP (Glu18-Ala493, AAH03782) , was fused with a 6X-His tag at the C-terminus and expressed in a mouse myeloma cell line, NS0. Molecular Mass: Based on N-terminal amino acid sequencing, the recombinant mouse PLTP starts at Glu 18 and has a calculated molecular mass of approximately 53.5kD. The recombinant mouse PLTP migrates as an approximately 70-85kD protein in SDS-PAGE under reducing conditions. Activity: Measured by its ability to inhibit LPS-induced TNF-alpha secretion by Raw264.7 cells. Storage and Stability: 12 months from date of receipt, 20 to 70°C as supplied., 1 month, 2 to 8°C under sterile conditions after reconstitution., 3 months, 20 to 70°C under sterile conditions after reconstitution., Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Schlagworte: Lipid transfer protein II, Phospholipid transfer protein
Hersteller: United States Biological
Hersteller-Nr: P4278-01

Eigenschaften

Konjugat: No
Immunogen: NS0, recombinant mouse PLTP, Glu 18-Ala 493, AAH03782.
Format: Purified

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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