PEDF, Recombinant, Human (Pigment epithelium-derived factor, Cell proliferation-inducing gene 35 pro

Pigment epithelium-derived factor (PEDF) is a 47kD secreted glycoprotein that belongs to the non-inhibitory serpin family group. PEDF is widely expressed in adult and foetal tissues, including brain, spinal cord, plasma, bone, prostate, pancreas, heart and lung. PEDF acts as an angiogenesis inhibitor with neurotrophic, immunomodulation and antitumor properties. It functions as anti-angiogenic agent by counterbalancing the proangiogenic effect of VEGF. PEDF is one of the most abundant proteins released by adipocytes and induces insulin resistance in adipocytes and human skeletal muscle cells. Recently, it has been reported that PEDF is sufficient to maintain the self-renewal of pluripotent human embryonic stem cells. Sequence: Human PEDF (aa20-418) is fused at the C-terminus of to a FLAG(R)-tag, expressed in CHO cells. Molecular Weight: ~47kD (SDS-PAGE), Endotoxin: <0.1EU/ug (LAL test, Lonza), Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.