Lysyl oxidase homolog 1 (Loxl1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 95-607aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: RQAPSLPLPG RVGSDTVRGQ TRHPFGFGQV PDNWREVAVG DSTGMARART SVSQQRHGGS ASSSVSASAF ATTYRQPSPY PQQFPYPQAP FVNQYENYDP ASRTYEQGYV YYRGAGGGMG AGAAAVASAG VIYPFQPRAR YEDYGGGGGE EQPEYPAQGF YPAPERPYVP QPQPQPQPQP QPQPQPSDGL DRRYSHSLYN EGTPGFEQAY PDPSTDVSQA PAGAGGTYGG AGDPRLGWYP PYAANVPPEA YVPPRAVEPQ PPFRVLEPPY LPVRSSDAPS QGGERNGAQQ GRLSVGSVYR PNQNGRGLPD LVPDPNYVQA STYVQRAHLY SLRCAAEEKC LASTAYAPEA TDYDLRVLLR FPQRVKNQGT ADFLPNRPRH TWEWHSCHQH YHSMDEFSHY DLLDASTGKK VAEGHKASFC LEDSTCDFGN LKRYACTSHT QGLSPGCYDT YNADIDCQWI DITDVQPGNY ILKVHVNPKY IVLESDFTNN VVRCNIHYTG RYVSTTNCKI VQS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Active on elastin and collagen substrates. Reference: An intron capture strategy used to identify and map a lysyl oxidase-like gene on chromosome 9 in the mouse.Wydner K.S., Kim Y., Csiszar K., Boyd C.D., Passmore H.C.Genomics 40:342-345(1997). Function: Active on elastin and collagen substrates.