Gingipain R2 (rgpB), partial, recombinant Porphyromonas gingivalis

Organism: Porphyromonas gingivalis (strain ATCC BAA-308 / W83). Source: Yeast. Expression Region: 230-736aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: YTPVEEKENG RMIVIVPKKY EEDIEDFVDW KNQRGLRTEV KVAEDIASPV TANAIQQFVK QEYEKEGNDL TYVLLVGDHK DIPAKITPGI KSDQVYGQIV GNDHYNEVFI GRFSCESKED LKTQIDRTIH YERNITTEDK WLGQALCIAS AEGGPSADNG ESDIQHENII ANLLTQYGYT KIIKCYDPGV TPKNIIDAFN GGISLANYTG HGSETAWGTS HFGTTHVKQL TNSNQLPFIF DVACVNGDFL YNVPCFAEAL MRAQKDGKPT GTVAIIASTI NQSWASPMRG QDEMNEILCE KHPNNIKRTF GGVTMNGMFA MVEKYKKDGE KMLDTWTVFG DPSLLVRTLV PTKMQVTAPA NISASAQTFE VACDYNGAIA TLSDDGDMVG TAIVKDGKAI IKLNESIADE TNLTLTVVGY NKVTVIKDVK VEGTSIADVA NDKPYTVAVS GKTITVESPA AGLTIFDMNG RRVATAKNRM VFEAQNGVYA VRIATEGKTY TEKVIVK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Its proteolytic activity is a major factor in both periodontal tissue destruction and in bacterial host defense mechanisms. Activates complement C3 and C5. Reference: "Comparative properties of two cysteine proteinases (gingipains R), the products of two related but individual genes of Porphyromonas gingivalis."Potempa J., Mikolajczyk-Pawlinska J., Brassell D., Nelson D., Thoegersen I.B., Enghild J.J., Travis J.J. Biol. Chem. 273:21648-21657(1998). Function: nan