FEN1 (human, recombinant)

Flap endonuclease 1 (FEN1), a RAD2 Class II nuclease, plays a crucial role in DNA repair, replication, and genomic stability. FEN1 removes the 5'-unannealed flap during Okazaki fragment processing in lagging strand DNA synthesis. Removal of the DNA flap is also critical in long-patch base excision repair and regulation of recombination. In yeast, FEN1 mutants display sensitivity to UV irradiation, deficient chromosome segregation, and cell cycle arrest in S phase. Furthermore, haploinsufficiency of FEN1 in mice leads to rapid tumor progression. FEN1 is overexpressed in many forms of cancer, including lung, gastric, prostate, pancreatic, brain, and breast.Synonyms: Flap Endonuclease 1, hFEN1. Purity: >85% estimated by SDS-PAGE. Source: Active recombinant C-terminal His-tagged protein expressed in E. coli. Amino Acids: 2-380 (full length). MW: 43.8 kDa. Formulation: (Request formulation change), 50 mM Tris, pH 8.0, containing 1 mM DTT and 10% glycerol.