FcgammaRIIIb/CD16b HNA-1b allotype (human, recombinant)

Low affinity immunoglobulin gamma Fc region receptor IIIb (FcgammaRIIIb) has roles in neutrophil adhesion, phagocytosis, and the respiratory burst. It is encoded by the FCGR3B gene in humans and is comprised of two immunoglobulin-like (Ig-like) extracellular domains that bind to IgG Fc and pentraxins, such as C-reactive protein. FcgammaRIIIb is synthesized as a precursor with a transmembrane domain and four-residue cytoplasmic domain that are cleaved in the endoplasmic reticulum, following which a glycosylphosphatidylinositol (GPI) anchor is added, which allows for membrane association of the mature protein. It can be expressed as three different allotypes known as human neutrophil antigens HNA-1a, HNA-1b, and HNA-1c, which are characterized by differential antibody binding affinities. FcgammaRIIIb is constitutively expressed in neutrophils and a subset of basophils and is shed from the cell surface as a soluble form via proteolytic cleavage during apoptosis or following neutrophil activation. Copy number variation of FCGR3B is associated with an increased risk of systemic lupus erythematosus (SLE) and primary Sjögren's syndrome. Cayman's FcgammaRIIIb/CD16b HNA-1b allotype (human, recombinant) protein consists of 176 amino acids and has a calculated molecular weight of 20 kDa.Synonyms: FCG3, FCGR3B, FCGR3B* 02, FCR-10, FCRIIIb. Purity: >95% estimated by SDS-PAGE. Source: Recombinant human FcgammaRIIIb expressed in E. coli. Amino Acids: 18-193. MW: 20 kDa. Formulation: (Request formulation change), Lyophilized from sterile 20 mM Tris (pH 7.4) with 50 mM sodium chloride. UniProt Accession #: O75015.