Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF)

Recombinant human epidermal growth factor(EGF) is a 6.3kD globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. Source: Recombinant human Epidermal Growth Factor, aa971-1023, expressed in E. coli. Molecular Weight: 6.2kD , Biological Activity: , ED50 is < 0.1ng/ml. ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells. Endotoxin: <1EU/ug (LAL) , Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.