Complement C1r-A subcomponent (C1ra), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 17-707aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SIYLPQKLYG EVTSPLYPKP YPSDLETTTV ITVPMGYRVK LVFWQFDVEP SEGCFYDYVK ISADKQTLGR FCGQLDSPLG NPPGSKEFMS QGNKMLLTFH TDFSNEENGT IMFYKGFLAY YQAVDLDECA SQPNSVEEGL QPRCQHLCHN YVGGYFCSCH PGYELQKDGQ SCQAECSSEL YTEPSGYVSS LEYPQPYPPD LRCNYSIRVE RGLTVHLKFL DPFEIDDHQQ VHCPYDQLQI YANGKNLGEF CGKQRPPDLD TSSNAVDLLF FTDESGDSRG WKLHYTTETI KCPQPKALDE FTIIQDPQPQ YQFRDYFIVT CKQGYQLMEG NQALLSFTAV CQNDGTWHRA MPRCKIKNCG QPQSLSNGDF RYITTKGVTT YEASIQYHCH EPYYKMLTRA GSSESMRGIY TCTAQGIWKN EEEGEKMPRC LPVCGKPVNP VTQKERIIRG QPARPGNFPW QAFTTTHGRG GGALLGDRWI LTAAHTIYPK HHNKENDNAN PKMLVFLGHT NVEQIKKLGH HPVRRVIIHP DYRQDEPNNF EGDIALLELE NSVTLGPELL PICLPDNETF YGQGLMGYVS GFGITEDKLA FDLRFVRLPV ADSEACQRWL QTKKDTSPFS QNMFCSGDPA VQQDACQGDS GGVFAVRDRN RDIWVATGIV SWGIGCGEGY GFYTKVLNYV DWIKKEMGDE N. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. Reference: "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007). Function: nan