Alginate Lyase, Recombinant, Azotobacter Vinelandii, aa24-374, His-Tag (AlgL)

Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. Splits ManA-ManA and ManA-GulA bonds, but not GulA-ManA or GulA-GulA bonds. Also cleaves acetylated residues (PubMed:9683471). May serve to degrade mislocalized alginate that is trapped in the periplasmic space (By similarity). Source: Recombinant protein corresponding to aa24-374 from Azotobacter vinelandii AlgL, fused to His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. AA Sequence: AEALVPP KGYYAPVDIR KGEAPACPVV PEPFTGELVF RSKYEGSDAA RSTLNEEAEK AFRTKTAPIT QIERGVSRMV MRYMEKGRAG DLECTLAWLD AWAEDGALLT TEYNHTGKSM RKWALGSLAG AYLRLKFSSS QPLAAYPEQA RRIESWFAKV GDQVIKDWSD LPLKRINNHS YWAAWAVMAA GVATNRRPLF DWAVEQFHIA AGQVDSNGFL PNELKRRQRA LAYHNYSLPP LMMVAAFALA NGVDLRGDND GALGRLAGNV LAGVEKPEPF AERAGDEDQD MEDLETDAKF SWLEPYCALY SCSPALRERK AEMGPFKNFR LGGDVTRIFD PAEKSPRSTV GKRD, Storage and Stability: May be stored at 4°C for short-term only. Aliqu