trFluor(TM) Eu succinimidyl ester

Ex: 346 nm. Em: 617 nm. Soluble in: DMSO. Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences. Our trFluorT Eu probes enable time-resolved fluorometry (TRF) for the assays that require high sensitivity. These trFluorT Eu probes have large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our trFluorT Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules. Moreover, our trFluorT Eu probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies.