Tide Fluor(TM) 1 azide (TF1 azide)

TF1 is designed to be a superior fluorophore alternative to EDANS. TF1 has (a). much stronger absorption, (b). much stronger fluorescence intensity, and (3). much less environment-sensitive fluorescence. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to EDANS. TF1-labeled peptides and nucleotides exhibit much stronger fluorescence and higher sensitivity than the ones labeled with EDANS. In pairing with our Tide Quencher(TM) 1 (TQ1), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF1 product is reactive to alkynes, and useful for click chemistry.