Protonex(TM) Red 600, SE

Ex: 575 nm. Em: 597 nm. Soluble in: DMSO. ProtonexT Red dye conjugates demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of ProtonexT Red dye conjugates. The fluorescence of ProtonexT Red dye conjugates dramatically increases as pH decreases from neutral to the acidic, making them a robust set of tools to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cells eliminates the wash steps. ProtonexT Red dye conjugates provide a set of powerful tools to study phagocytosis and to monitor acidic cell compartments such as endosomes and lysosomes. ProtonexT Red dye conjugates are non-fluorescent outside the cells, but fluoresce brightly red in acidic compartments (such as phagosomes, lysosomes and endosomes). This ProtonexT Red SE can be readily used to make a variety of bioconjugates for imaging or flow applications, enabling the specific detection of phagocytosis and endocytosis with reduced signal variability and improved accuracy. These conjugates can be also used for multiplexing cell functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. ProtonexT Red has the spectral properties similar to those of Texas Red, making the common filter set of Texas Red readily available to the assays of ProtonexT Red.