PhosLite(TM) Green

This water-soluble PhosLite(TM) Green substrate generates a bright and photostable yellow-green fluorescent precipitate at the site of phosphtase activity. Its fluorescent precipitate has several unique an extremely large Stokes shift and high photostability, making this substrate a superior fluorescent version of 5-Bromo-4-chloro-3-indolyl phosphate (BCIP), an chromgenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It can be used in immunoblotting, in situ hybridization, and immunohistochemistry, but does not require the addition of nitro blue tetrazolium chloride (NBT). Alkaline phosphatase is commonly conjugated to secondary antibodies.