LDS 751

LDS 751 is a fluorescent cell-permeant nucleic acid stain that can be well excited with 488 nm laser line although it has a peak excitation at approx. 543 nm on dsDNA. It might be an alternative to DRAQ 5(TM), useful in multicolor analyses due to its long wavelength emission maximum (approx. 712 nm). Upon binding to dsDNA of LDS 751 has approx. 20-fold fluorescence enhancement. It has been reported that LDS-751 is excluded from the nucleus and binds the polarized membranes of mitochondria. Thus cautions need be taken when LDS 751 is used for analyzing live cells and LDS-751 fluorescence as being indicative of nuclear status. Thiazole orange (an RNA dye) and LDS-751 (a DNA dye) composition has been used to separate reticulocytes from other cell populations. LDS 751 has been also used to separate red blood cells from nucleated cells.