Coelenterazine 400a

Coelenterazine 400a is a bisdeoxy derivative of coelenterazine (Cay-16123) that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc). It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h (Cay-16894), which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols. Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10. BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.Formal Name: 6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one. CAS Number: 70217-82-2. Molecular Formula: C26H21N3O. Formula Weight: 391.5. Purity: >95%. Emission: 395 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: Ethanol: 0.5 mg/ml *, Methanol: 0.5 mg/ml *. lambdamax: 248, 361, 432 nm. SMILES: O=C1C(CC2=CC=CC=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31. InChi Code: InChI=1S/C26H21N3O/c30-26-23(17-20-12-6-2-7-13-20)28-25-22(16-19-10-4-1-5-11-19)27-24(18-29(25)26)21-14-8-3-9-15-21/h1-15,18,27H,16-17H2. InChi Key: MWOYPGFRXPPBOX-UHFFFAOYSA-N.