Amplite(TM) Blue

A variety of substrates are available for detecting peroxidase activity in ELISA assays. Colorimetric HRP substrates (e.g., TMB, OPD and ABTS) have been widely used for years. Each of these substrates varies greatly with respect to its performance characteristics such as detection sensitivity, working range and attainable signal-to-noise ratios. Our Amplite(TM) Blue peroxidase substrate is a soluble fluorogenic substrate for detection of peroxidase activity. Amplite(TM) Blue allows rapid HRP detection assays to be performed with greater sensitivity than the colorimetric HRP substrates (such as TMB and OPD). The fluorescent product of Amplite(TM) Blue does not photobleach. Amplite(TM) Blue exhibits a flat baseline in assays, which facilitates low-level detection sensitivity and allows for high signal-to-noise ratios.