HEPES Sodium Salt (N-[2-Hydroxyethyl] piperazine-N'- [2-ethanesulfonic acid] sodium salt)

HEPES Sodium is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes. Synonym: N-[2-Hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid] sodium salt, CAS No: 75277-39-3, Molecular Formula: C8H17N2O4SNa, Molecular Weight: 260.3, Appearance: White, crystalline powder, Purity: >99.0% (Dried basis), pH (1%): 10.0 ± 1.0, pKa: 7.5 ± 0.2, Water: <1.5%, Heavy Metals: <0.001%, Chloride: <300ppm, RNase, DNase, Protease: None Detected, Endotoxin: < 2EU/mg, Country of Origin: USA