Y-27632 (hydrochloride)

The ROCK family of Rho-associated serine-threonine protein kinases is known to play an important role in Rho-mediated cell adhesion and smooth muscle contraction. Y-27632 is a potent, ATP-competitive inhibitor of ROCKs including p160ROCK (Ki = 140 nM) and ROCK2 (IC50 = 800 nM). It also inhibits PRK2 with an IC50 value of 600 nM. One-hour treatment with 10 µM Y-27632 blocks apoptosis of dissociated cultured human embryonic stem cells, increasing cloning efficiency by 25% and sustaining survival up to 30 passages.Formal Name: trans-4-[(1R)-1-aminoethyl]-N-4-pyridinyl-Cyclohexanecarboxamide, dihydrochloride. CAS Number: 129830-38-2. Molecular Formula: C14H21N3O . 2HCl. Formula Weight: 320.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: 30 mg/ml, Ethanol: 1 mg/ml, PBS (pH 7.2): 100 mg/ml, Water: 30 mg/ml. lambdamax: 270 nm. SMILES: O=C([C@H]1CC[C@@]([C@H](N)C)([H])CC1)NC2=CC=NC=C2.Cl.Cl. InChi Code: InChI=1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13,,/h6-12H,2-5,15H2,1H3,(H,16,17,18),2*1H/t10-,11-,12-,,/m1../s1. InChi Key: IDDDVXIUIXWAGJ-LJDSMOQUSA-N.