HI TOPK 032

HI TOPK 032 is an inhibitor of lymphokine-activated killer T cell-originated protein kinase (TOPK), blocking phosphorylation of the substrate histone H2AX with an IC50 value of ~2 µM and providing complete inhibition at 5 µM. It also inhibits checkpoint kinase 1 (Chk1, IC50 = 9.6 µM). In addition, HI TOPK 032 inhibits MEK1, achieving 40% inhibition at 5 µM, but it does not alter the activities of ERK1, JNK1, or p38 MAPK at 2 µM. HI TOPK 032 decreases the growth of colon cancer and glioma initiating cells in vitro and suppresses tumor growth in vivo.Formal Name: N-(12-cyanoindolizino[2,3-b]quinoxalin-2-yl)-2-thiophenecarboxamide. CAS Number: 487020-03-1. Molecular Formula: C20H11N5OS. Formula Weight: 369.4. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: :, DMSO: 3mg/mL (warmed). lambdamax: 219, 272, 302, 369, 496 nm. SMILES: O=C(C1=CC=CS1)NC2=CC3=C(C#N)C4=NC5=CC=CC=C5N=C4N3C=C2. InChi Code: InChI=1S/C20H11N5OS/c21-11-13-16-10-12(22-20(26)17-6-3-9-27-17)7-8-25(16)19-18(13)23-14-4-1-2-5-15(14)24-19/h1-10H,(H,22,26). InChi Key: BCSBXWKRZUPFHW-UHFFFAOYSA-N.