(+)-Biotin N-hydroxysuccinimide ester

Biotin succinimidyl ester is the most popular amine-reactive biotinylating agent for modifying proteins, peptides, antibodies, oligonucleotides, microbeads and other biological molecules. It forms irreversible amide bonds. Typically coupled to primary amine in the pH range 6.5-8.5. This cell-permable activated biotin derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. The resulting biotinylated derivatives have been widely used in streptavidin or avidin systems and labeling methods, with many applications in immunology and histochemistry. It is used to selectively label Escherichia coli cell envelope proteins in vivo. It preferentially labels outer membrane, periplasmic, and inner membrane proteins as well as a specific inner membrane marker protein (Tet-LacZ).