Aminopropargyl dCTP [5-Propargylamino-2'-deoxycytidine-5'-triphosphate]

Aminopropargyl dCTP [5-Propargylamino-2'-deoxycytidine-5'-triphosphate]
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
ABD-17050 10 µmol -

3 - 8 Werktage*

580,00 €
 
The amine-modified deoxycytidine 5'-triphosphates (such as... mehr
Produktinformationen "Aminopropargyl dCTP [5-Propargylamino-2'-deoxycytidine-5'-triphosphate]"
The amine-modified deoxycytidine 5'-triphosphates (such as 5-propargylamino-2'-deoxycytidine-5'-triphosphate) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminopropargyl dCTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminopropargyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled nucleotides that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments
Hersteller: AAT Bioquest
Hersteller-Nr: 17050

Eigenschaften

Anwendung: Two-step DNA labelling, FISH, Microarrays
MW: 608,15 D
Format: Desiccated

Datenbank Information

Handhabung & Sicherheit

Lagerung: -20°C (protect from light)
Versand: -20°C (International: -20°C)
Signalwort: Warning
P-Sätze: P304, P312
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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