NCI-H1703 cell line

Background: This cell line was isolated from the same patient as was NCI-H1693. Species: Homo sapiens, Human. Age: 54 years adult. Tissue: Lung. Morphology: Epithelial. Growth properties: Adherent. Biosafety: 1. Medium: RPMI-1640+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Ratio: 1:3-1:4. Renewal: Every 2 to 3 days. Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase. Culture conditions: Atmosphere: Air, 95%, CO2, 5%, Temperature: 37 °C.