Hela S3 cell line

Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
E-EP-CL-0350.1 1 MCells (1×10^6 cells/vial) - -

7 - 16 Werktage*

696,00 €
 
Background: HeLa S3 is a clonal derivative of the parent HeLa line. S3 was cloned in 1955 by T.T.... mehr
Produktinformationen "Hela S3 cell line"
Background: HeLa S3 is a clonal derivative of the parent HeLa line. S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura. Species: Homo sapiens, human. Age: Female,31 years. Tissue: Cervix. Morphology: Epithelial. Growth properties: Adherent. Doubling time: ~48 hours. Biosafety: 2. Medium: Ham's F-12K+10% FBS+1% P/S. Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Ratio: 1:2-1:5. Renewal: Every 2 to 3 days. Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase. Culture conditions: Atmosphere: Air, 95%, CO2, 5%, Temperature: 37 °C.
Hersteller: Elabscience
Hersteller-Nr: E-EP-CL-0350

Eigenschaften

Reaktivität: Human

Datenbank Information

Handhabung & Sicherheit

Lagerung: Liquid nitrogen
Versand: -80°C (International: -80°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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